Use the eyedropper to place a drop of water on the center of a microscope slide 2. How is a prepared slide different from a wet mount. Aug, 2010 in a wet mount, the specimen is suspended in a drop of liquid usually water located between slide and cover glass. This article shows you how, using powerpoint 2002 for windows xp. This page provides step by step instructions on slide preparation as well as videos at the bottom of page. Wet mount advantages are that they are easy and cheap to make, easy to correct if you mess them up, and the distilled water offers a perfectly clear view of the specimen. Saline wet mount is used for the detection of trophozoites and cysts of protozoa, and eggs and larvae of helminthes.
Back in the days before digital photography was the norm, there were generally 2 methods of processing film. How to make a wet mount slide ayushisinhamicroscopy. One of my personal tricks is to add a very light background with some subtle texture to give the slides more depthhead over to subtle patterns for inspiration and free downloadable backgrounds. This is a general video on how to prepare a wet mount slide. Place and view your specimen on the microscope and use the medium power lens to view for more focus. This is a short how to video on creating microscope slides. Place your specimen in the middle of a clean and dry slide 3. Choose from 47 different sets of steps for making a wet mount slide flashcards on quizlet. This skill is only developed with practice and by following precise instructions. Direct saline iodine wet mount for diagnosis of intestinal.
How to make a wet mount microscope slide techwalla. Cut out a letter e from a newspaper or other printed page. Wet mount slide definition of wet mount slide by the. To begin creating the template that youll use in your presentation, do the following. Carefully clean the slide and cover glass with a tissue. Preparing wet mount slides ppt download slideplayer. Answer to make a wet mount slide first get a clean. Jan 10, 2016 wet mount hanging drop mount the rate of movement slows down because the wet mount slide dries due to the heat from the light of the microscope. The water refractive index of the water improves the image quality and also supports the specimen. To remove an unwanted, default placeholder from a layout, in the slide thumbnail pane, click the slide layout that contains the placeholder, click the border of the placeholder in the presentation window, and then press delete to add a text placeholder, in the slide thumbnail pane, click. Place the slide on the stage and view it first with the red banded objective. Temporary slides are made to be viewed for the day, and then they are cleaned and usually discarded.
Procedure for making a wet mount ppt video online download. Here is the procedure for aking a wet mount slide of a letter. Make them more exciting using layered, animated images. Vaginitis differentiation vaginitis curriculum pseudohyphae or spores if non albicans species koh wet mount clue cells 20%, nofew wbcs few wbcs motile flagellated protozoa, many wbcs lactobacilli nacl wet mount positive negative often positive negative koh whiff test 4. A blank slide master for title and text layouts is available. The infographic 4 steps pencil banner powerpoint diagram is a professional presentation featuring a column with banner signs created from a hb wooden pencil. As students view their slide with specimens from the pond, i want them to make connections about the biodiversity of pond life. What is difference between wet mount and permanent slide. With your scissors cut out the letter e from the newsprint.
You probably dont know what the organism is and will probably have to identify it. Topics include focusing, field of view, resolution, making a wetmount slide, and measuring with a ruler. Be sure to handle the slide and cover glass carefully, since they are easily broken. Disadvantages are that the slip cover can move slightly and blur the image and if left for too long, the water dries up and the slide falls apart. Use tweezers to place your specimen on the drop of water.
Topics include focusing, field of view, resolution, making a wet mount slide, and measuring with a ruler. We have designed this ppt template that fits ideal for your next businesses presentations. Wet mount microscope lab preparing a wet mount of the letter e. Handle the slide and cover glass by the edges to avoid getting finger prints on the glass. It is particularly useful for detection of live motile. Draw what you see and make notes comparing this slide to your first slide.
Cytology is inexpensive and readily available and may allow rapid confirmation and identification of an infectious agent. How to prepare a wet mount slide of eukaryotic cells. Prints were developed on a sheet of photo paper, while slides were small. A wet mount is a slide that you prepare on your own, using a little bit of water to make things easier. Microscope slides preparation styles and techniques. Place the edge of the cover slip on one side of the water drop. Below you will see the procedure for making temporary. In this exercise you will make a wet mount on a microscope slide and look at the cells of the onion membrane magnified by the high power, compound microscope.
The sample to be viewed floats in a layer of water which is between the slide and the cover glass. In this hands on lesson students make their own wet mount slide of an onion and are able to identify the cells in it such as, the nucleus. The water should just fill the space between the cover slip and the slide. Objective lenses are the primary optical lenses on a microscope standard objectives include 4x, 10x, 40x and 100x although different power. Unlike a wet mount slide, there cannot be excessive amounts of water, and the specimen itself must be sliced very thin. Place the other piece of hair on the other slide and make a wet mount. The wet mount contains live organisms that were collected from whatever substance your teacher wants you to analyze. The rate of movement is constant because the petroleum jelly keeps the hanging drop mount hydrated. Making a wet mount slide use a dropper to place comparing onion and elodea cells look for. Wet mount slide synonyms, wet mount slide pronunciation, wet mount slide translation, english dictionary definition of wet mount slide. Use the lens paper to clean a glass slide and coverslip. In this microscope instructional activity, students label the parts of a microscope, they calculate the powers of magnification for each lens on the scope and they answer questions about making a wet mount slide. Holding the cover slip at a 45 degree angle, lower it slowly. Preparing wet mount slides presentation transcript.
How to prepare a wet mount slide of eukaryotic cells page 2. This kind of mount is usually used for inanimate objects or things that do not need water to live. Draw what you saw at three 3 different power magnifications. Oct 23, 2014 this is a general video on how to prepare a wet mount slide. To begin, drop a few drops of water onto the slide. First add a few drops of water or solution on the microscope slide to avoid dryness and wilting. Now, take a clean coverslip and put it over the slide you made. Ive identified seven different powerpoint slide designs to inspire you do add others in the comments. Use the tweezers to position the letter e in the center of the drop.
This will help to prevent air bubbles from getting trapped under the cover slip. Steps to making a wet mount slide flashcards quizlet. After testing, i found that i can laboriously copy and paste all onscreen elements and notes to a new ppt presentation. Making a permanently mounted slide of an insect wing. Wet mount hanging drop mount the rate of movement slows down because the wet mount slide dries due to the heat from the light of the microscope. Slides using the microscope focusing the microscope preparing wet mounts. The main methods of placing samples onto microscope slides are wet mount, dry mount, smear, squash and staining. Ppt prepare a wet mount powerpoint presentation, free download. Have a clean, dry microscope slide and cover slip in front of you. Here it will prevent the onion from wilting and drying.
Clean a microscope slide and prepare a wet mount of the letter, using the procedure described below. Cytologic examination of exudates, blood film, tissue imprint, aspiration biopsy, or wet mount of hair is indicated when bacterial and fungal diseases and occasionally rickettsial and viral diseases are suspected. Place 1 or 2 drops of water in the middle of the slide. Making a wet mount for microscopy microbehunter microscopy. Experimental preparation of temporary mounts of an onion peel. Wet mount slide definition of wet mount slide by the free. Collect a drop of stain with an eye dropper or pipette. Learners observe the cells of an onion by wet mounting it and viewing it in a microscope. Put a drop of stain on an outer edge of your cover slide. I see no place to renumber or reset the slide numbers. How to prepare wet mount slides nova scotia department. In a wet mount, the specimen is suspended in a drop of liquid usually water located between slide and cover glass.
To start, go to the view menu to master slide master and create your template. Switch to medium power or high power as necessary to easily visualize the inside of the onion root cells. An onion, glass slide, watch glass, coverslip, forceps, needles, brush, blade, filter paper, safranin, glycerine, dropper. Making a wet mount microscope slide microbehunter microscopy. Learn vocabulary, terms, and more with flashcards, games, and other study tools. How to prepare a dry and wet mount slide dry mount. Remove one of the layers and peel off a piece of the clear membrane with the.
An onion is made of layers, each separated by a thin skin or membrane. Infographic 4 steps pencil banner powerpoint diagram. Take a small slice of onion and gently pull apart a layer of the onion skin. This will help draw the stain under the cover and across the specimen. This is one of the more challenging parts of making a prepared mount slide. Or, if you want your new slide to have the same layout that the slide before. Place one drop of water in the middle of the slide. Each banner is numbered and labeled with text placeholders. School sciencehow to prepare an onion cell slide wikibooks. Learn steps for making a wet mount slide with free interactive flashcards. In contrast to permanently mounted slides, wet mounts can not be stored over extended time periods, as the water evaporates.
On the file tab, click save as, in the file name box give your template a name, and then in the save as type box, select powerpoint template. Jan 29, 2010 the general procedure of making a wet mount place a drop of water on the center of the slide. Well be making a wet mount slide for this experiment. To customize the slide master and associated layouts, do one or more of the following. A new slide will be placed below whatever slide is active in your slide pane. After that, use a medicine dropper, and carefully place one drop of water over the specimen.
Sep 26, 2011 this is a short how to video on creating microscope slides. Place a piece of napkin or paper towel against the opposite side of your cover slip, right up against the edge. Just download the fonts, install them on your computer, and prettify your presentations in no time. To make a wet mount slide, first get a clean microscope slide, and place a specimen on it. Draw what you see and make note comparing this slide to your first and second slide. The target audience is middle school science students. Wet mounts are commonly used in the science of biology. Using low magnification on the microscope, focus on the root cells. Aug 29, 2010 wet mount advantages are that they are easy and cheap to make, easy to correct if you mess them up, and the distilled water offers a perfectly clear view of the specimen. Place the edge of a coverslip on the slide so that it touches the edge of the water. Click a heading below to open the section and see the instructions. Sealed microscope slides diy semipermanent microscope slides.
The powerpoint revolution has sparked the evolution of different styles of powerpoint slide design. Saline wet mount is made by mixing a small quantity about 2 mg of faeces in a drop of saline placed on a clean glass slide. Preparing a wet mount of a specimen is the technique typically used to view plant and animal cells. If youre just getting started with creating nonbullet point slides, i recommend the assertionevidence or presentation zen style. Making mitosis slides continued 3 21 linn sientii n ll igts eseed 16. Place one end of the cover slip on the slide and slowly lower the other end. When you add a slide to your presentation, you can choose a layout for the new slide at the same time. Wet mount medical definition merriamwebster medical dictionary. How to prepare a dry and wet mount slide cells for dummies. Preparing a wet mount of a specimen is the technique typically used to view plant and animal cells using a microscope. This is a simple lab where students learn how to properly use the microscope. A mounting medium is necessary if you are to obtain a clear image of the specimen. Study all of the squashed tissue to locate cells in each stage of the cell cycle.
It is also possible to first place the specimen on the slide, but small specimens usually separate more easily from the tweezers or needle if dipped into the drop of water. This is an important skill to develop in order to be able to study specimens with a microscope. Hold the cover slip by its edges, at an angle of about 45 degrees to the surface of the slide. Using an eyedropper put a drop of water on the sample. Below you will see the procedure for making temporary slides. Steps to making a wet mount slide, microscope parts, care of microscope.
This is part of our collection of exclusives powerpoint presentation templates or if you prefer, you might take a look at many other powerpoint design templates that we have designed for you, we have many. Are your powerpoint presentations putting your audience to sleep. Filled calibrated wells or flasks are viewed quickly without preparing or clipping individual slides to the microscope stage, making this especially useful in. Use a medicine dropper to put a small drop of water in the centre of the slide. A wet mount link is simply when the specimen is suspended in liquid. Finally, put the slide under a microscope and see what happens. To prepare a stained temporary mount of an onion peel and to record observations and draw labeled diagrams. Wet mount medical definition merriamwebster medical. The top 7 powerpoint slide designs speaking about presenting.
I model each step for the students since for most of the students, this is the first slide they have ever created. Get a cover slip and hold it by its edges and gently place it over your specimen 4. With everything still selected, right click and choose send to back. Now each time you insert new slide your slide will use the master layout you just designed. Mar 09, 2010 vaginitis differentiation vaginitis curriculum pseudohyphae or spores if non albicans species koh wet mount clue cells 20%, nofew wbcs few wbcs motile flagellated protozoa, many wbcs lactobacilli nacl wet mount positive negative often positive negative koh whiff test 4. Using your eyedropper, place a drop of water on the edge of the cover slip where it touches the.
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